Analysis of complex mixtures of substances ― especially biologically relevant materials ― typically requires separation of the components. Many methods have been developed to accomplish this task, and the method used is dependent on the types of substances which comprise the mixture. All these methods rely on the magnification of potential differences in the strength of intermolecular interactions.
The content in this category covers separation and purification methods including extraction, liquid and gas chromatography, and electrophoresis.
| Topic |
Biochemistry |
Biochemistry: A Short Course |
Fundamentals of Biochemistry |
Karp’s Cell and Molecular Biology |
Separations and Purifications (OC, BC)*
- Extraction: distribution of solute between two immiscible solvents
- Distillation
- Chromatography
- Basic principles involved in separation process
- Column chromatography, gas-liquid chromatography
- High pressure liquid chromatography
- Paper chromatography
- Thin-layer chromatography
- Separation and purification of peptides and proteins (BC)
- Electrophoresis
- Quantitative analysis
- Chromatography
- Size-exclusion
- Ion-exchange
- Affinity
- Racemic mixtures, separation of enantiomers (OC)
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- Ch. 3 Exploring Proteins and Proteomes, pp. 69-105
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- Ch. 5 Techniques in Protein Biochemistry, pp. 75-98
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- Ch. 5 Proteins: Primary Structure: Section 2. Protein Purification and Analysis, p. 99-108
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- Ch. 18 Techniques in Cell and Molecular Biology, pp. 692-716
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